Received 22 May 2008; received in revised form 18 June 2008; accepted 13 August 2008.
Objectives/Hypothesis
To determine the types and localization of connexins within the rat larynx.
Study Design
Quantitative real time polymerase chain reaction (qRT-PCR) of the epiglottis and laryngeal mucosa was used to identify connexins (Cx). Immunohistochemical labeling was then used to localize the Cxs within the larynx.
Methods
Twelve larynges from 3 to 4 month old Fisher-344 rats were used. RNA was extracted (N = 8) and cDNA produced. Primers for Cx26, Cx30, Cx32, Cx37, Cx40, and Cx43 were added and qRT-PCR performed. Others larynges were serially sectioned for immunohistochemistry.
Results
qRT-PCR revealed Cx43, Cx32, and Cx30 within the epiglottis and Cx43 in the vocal folds and Cx43 and Cx32 within the subglottic mucosa. Immunohistochemical staining confirmed these results.
Conclusion
The rat epiglottis is rich in Cx43, Cx32, and Cx30 whereas the vocal folds contain Cx43 and the subglottic mucosa Cx43 and Cx32. Their localizations suggest involvement in secretion for protective purposes and they may play a key role in laryngeal pathoses.
aDepartment of Otolaryngology and Communication Sciences, SUNY Upstate Medical University, Syracuse, New York
bDepartment of Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, New York
Corresponding author: Michael J. Lyon, PhD, Department of Otolaryngology, SUNY Upstate Medical University, 750 East Adams St, Syracuse, NY 13210
ABSTRACT