Transforming growth factor β3 increases chondrocyte proliferation and decreases apoptosis in murine cricoid cartilage in vitro
Presented during the Combined Otolaryngology Spring Meeting at the 22nd annual meeting of the American Society of Pediatric Otolaryngology, San Diego, California, April 28, 2007.
Received 21 June 2007; received in revised form 3 November 2007; accepted 6 November 2007.
Objective
To determine if the luminal epithelium and/or exogenous transforming growth factor beta (TGFβ) affects growth of the cricoid.
Design
Subglottises from 20 neonatal mice were subdivided into four groups: A, five subglottises with luminal epithelium grown in basic medium; B, five epithelium-free subglottises in basic medium; C, five epithelium-free subglottises in basic medium with supplemental TGFβ1, and D, five epithelium-free subglottises in basic medium with supplemental TGFβ3.
Results
Groups A and D demonstrated the greatest luminal area expansion. Group A rings demonstrated statistically higher chondrocyte proliferation than Groups B and C and lesser amounts of luminal apoptosis. Groups B and C rings demonstrated the least amount of cell proliferation, and greater luminal apoptosis relative to Group A. Groups A and D rings had similar apoptotic and proliferative results.
Conclusions
Luminal epithelium exerts influence over the cricoid by increasing chondrocyte proliferation and decreasing the relative proportion of luminal chondrocytes that undergo apoptosis. Exogenous TGFβ3, not TGFβ1, also increases chondrocyte proliferation within the cricoid and appears to influence apoptosis as well.
Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA.
Corresponding author: Leila Mankarious, MD, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA, 02114.
ABSTRACT